US4588492A | 寵物協尋網
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PublicationofUS4588492A.2004-05-07.Anticipatedexpiration...3isaschematiclongitudinalcross-sectionoftherotatingIEFapparatusshowninFIG.
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This invention relates to the separation and/or purification of biological materials and, more particularly, to apparatus for isoelectric focusing. The United States Government has rights in this invention pursuant to NASA contract NAS 8-32950.
BACKGROUND OF THE INVENTIONIsoelectric focusing ("IEF") also sometimes called electrofocusing, is an electrophoretic technique that is recognized as being a powerful method for the analysis and micropreparative separation and purification of various biological materials, including proteins, peptides, nucleic acids, viruses, and even some living cells of cell organelles. The principle of IEF is based on the fact that certain biomaterials, such as those listed above, are amphoteric in nature, i.e. are positively charged in acidic media and negatively charged in basic media. At a particular pH value, called the isoelectric point, they have a zer...
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